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Water Use In Cryobiology
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| Deep freezing provides indefinite preservation of living tissue much like blood storage preserves blood cells. In cryobiology tissues are washed in an isotonic solution made with ultrapure water. They are frozen slowly and stored in liquid nitrogen.Tissue stored this way include skin cornea bone marrow blood vessels and human tumors. |
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Preservation through freezing has become more commonplace in the last 10 years. It was necessitated by the need for genetic stability in cancer research.The freezing preparation involves immersing fragments of tissue in glass ampoules containing a nutrient medium and glycerol in ultrapure water. After this the ampoules are sealed and cooled at a rate of 1oC per minute from ambient room temperature to -25oC. Then they are rapidly cooled to -70oC. This prevents ice crystal growth. Next they are stoed in liquid nitrogen at -195oC. When the tissue is ready to be used the ampoules are thawed rapidly using a water bath at 37oC. The tissue is then rinsed several times in a saline solution made with biopure water to remove the glycerol before being used. |
| Preserving tumor tissue for cancer research using this method does not alter a tumors morphology transplantablity or sensitivity to treatment with known growth inhibitors. In the first transplant generation thawed tumors grow more slowly than non forzen tumors. After several generations they will recover their normal growth rate. Lastly there is no measurable difference in viability between frozen and non frozen tumors. |
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Primer: Water Use In Research And Medicine
Water The Cleansing Agent
Water Use In Tissue Cell Culture
Water Usage In Pharmaceuticals
Pyrogens In Research And Medicine
Water Use In Blood Fractionation
Water Use In Cryobiology
General Use of Water in Research and Medicine
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